Afrika swine fever virus PCR ikiti yokubona

Inkcazelo emfutshane:

Le khithi isebenzisa indlela ye-PCR yexesha lokwenyani lokubona i-DNA ye-Africa swine fever virus (ASFV) kwizinto zesifo se-tissue ezifana neetoni, i-lymph nodes kunye ne-spleen kunye nezixhobo zezifo ezingamanzi ezifana nogonyo kunye negazi lehagu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

Afrika ihagu fever virus ikiti yokufumanisa iPCR (Lyophilized)

Ubungakanani

48tests/kit, 50tests/kit

Ukusetyenziswa okucetywayo

Le khithi isebenzisa indlela ye-PCR yexesha lokwenene le-fluorescent ukubona i-DNA yeIntsholongwane yehagu yaseAfrika (ASFV)kwimathiriyeli yesifo sethishu ezifana neetoni, i-lymph nodes kunye ne-spleen kunye nezixhobo zesifo solwelo ezifana nogonyo kunye negazi lehagu.Ilungele ukufunyaniswa, ukuxilongwa kunye nophando lwe-epidemiologicalIntsholongwane yehagu yaseAfrika.Ikhithi YINKQUBO YEPCR EZILUNGILEYO ZONKE (Lyophilized), equlethe i-enzyme yokukhulisa i-DNA, i-reaction buffer, iiprimers ezithile kunye ne-probes ezifunekayo ukubhaqwa kwe-PCR yefluorescent.

Imixholo yeMveliso

Amacandelo Iphakheji iinkcukacha Isithako
Umxube we-ASFV PCR 1 × ibhotile (Lyophilized powder)  50Uvavanyo ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase
6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) 48Uvavanyo
Ulawulo Olulungileyo 1 * 0.2ml ityhubhu (lyophilized)  10Uvavanyo

I-Plasmid equlethe amaqhekeza athile e-ASFV

Isisombululo sokunyibilika 1.5 ml Cryotube 500uL /
Ulawulo olubi 1.5 ml Cryotube 200uL 0.9%NaCl

Ugcino kunye noBomi beShelufu

(1)Ikhithi inokuthuthwa kubushushu begumbi.

(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.

(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.

4

Izixhobo zomculo

GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.

Umzobo wokuSebenza

a) Uguqulelo lwebhotile:

1

b) 8 inguqulelo yetyhubhu enemicu entle:

2

Ukwandiswa kwePcr

KucetyiswaUkumisela

Inyathelo Umjikelo Iqondo lobushushu (℃) Ixesha Itshaneli yeFluorescence
1 1 95 2 min  
2 40 95 5s  
60 10s Qokelela i-FAM fluorescence

*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.

Iziphumo zovavanyo lokutolika

Umjelo

Ukutolikwa kweziphumo

FAM Channel

Ct≤35

ASFV Positive

Undet

ASFV Negative

35

Iziphumo ezirhanelwayo, phinda uvavanye*

*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.


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