Swine fever virus RT-PCR Detection Kit

Inkcazelo emfutshane:

Le khithi isebenzisa indlela yokwenyani ye-RT-PCR yokubona i-RNA yentsholongwane ye-Swine fever (CSFV) kwimathiriyeli yesifo se-tissue ezifana neetoni, ii-lymph nodes kunye ne-spleen kunye nezixhobo zesifo ezingamanzi ezifana nogonyo kunye negazi lehagu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-Swine fever virus RT-PCR Detection Kit (Lyophilized)

Ubungakanani

48tests/kit, 50tests/kit

Ukusetyenziswa okucetywayo

Le khithi isebenzisa indlela yokwenyani ye-RT-PCR yokubona i-RNA yentsholongwane ye-Swine fever (CSFV) kwimathiriyeli yesifo se-tissue ezifana neetoni, ii-lymph nodes kunye ne-spleen kunye nezixhobo zesifo ezingamanzi ezifana nogonyo kunye negazi lehagu.Ilungele ukufunyaniswa, ukuxilongwa kunye nophando lwe-epidemiological yentsholongwane ye-Swine fever.Ikhithi yi-ALL-READY PCR SYSTEM (i-Lyophilized), equlethe i-enzyme yokukhulisa i-DNA, i-reverse transcriptase, i-reaction buffer, i-primers ethile kunye ne-probes efunekayo ukufunyanwa kwe-PCR ye-fluorescent.

Imixholo yeMveliso

Amacandelo Iphakheji iinkcukacha Isithako
CSFV PCR Mix 1 × ibhotile (Lyophilized powder)  50Uvavanyo ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase
6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) 48Uvavanyo
Ulawulo Olulungileyo 1 * 0.2ml ityhubhu (lyophilized)  10Uvavanyo

I-Plasmid equlethe amaqhekeza athile e-CSVV

Isisombululo sokunyibilika 1.5 ml Cryotube 500uL /
Ulawulo olubi 1.5 ml Cryotube 200uL 0.9%NaCl

Ugcino kunye noBomi beShelufu

(1)Ikhithi inokuthuthwa kubushushu begumbi.

(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.

(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.

4

Izixhobo zomculo

GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.

Umzobo wokuSebenza

a) Uguqulelo lwebhotile:

1

b) 8 inguqulelo yetyhubhu enemicu entle:

2

Ukwandiswa kwePcr

KucetyiswaUkumisela

Umjikelo Iqondo lobushushu (℃) Ixesha Itshaneli yeFluorescence
1 1 50 8min  
2 1 95 2 min  
3 40 95 5s  
60 10s Qokelela i-FAM fluorescence

*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.

Iziphumo zovavanyo lokutolika

Umjelo

Ukutolikwa kweziphumo

FAM Channel

Ct≤35

CSFV Positive

Undet

CSFV Negative

35

Iziphumo ezirhanelwayo, phinda uvavanye*

*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.


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  • Okulandelayo:

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