I-Norovirus (GⅠ) RT-PCR Ikhithi yokuFumana

Inkcazelo emfutshane:

Ifanelekile ukufunyanwa kwe-Norovirus (GⅠ) kwi-shellfish, imifuno eluhlaza kunye neziqhamo, amanzi, ilindle, ukuhlanza kunye neminye imizekelo.Ukukhutshwa kwe-Nucleic acid kufuneka kuqhutywe ngekhithi ye-nucleic acid extraction okanye indlela ye-pyrolysis ngokuthe ngqo ngokweentlobo ezahlukeneyo zesampulu.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-Norovirus (GⅠ) RT-PCR IKit yokuFumana (Lyophilized)

Ubungakanani

48tests/kit, 50tests/kit

Ukusetyenziswa okucetywayo

Ifanelekile ukufunyanwa kwe-Norovirus (GⅠ) kwi-shellfish, imifuno eluhlaza kunye neziqhamo, amanzi, ilindle, ukuhlanza kunye neminye imizekelo.I-nucleic acid extraction kufuneka iqhutywe nge-nucleic acid extraction kit okanye indlela ye-pyrolysis ngokuthe ngqo ngokweentlobo ze-specimen ezahlukeneyo. kunye neeprobes ezifunekayo ukufunyanwa kwe-PCR yefluorescent.

Imixholo yeMveliso

Amacandelo Iphakheji iinkcukacha Isithako
Norovirus (GⅠ) PCR Mix 1 × ibhotile (Lyophilized powder)  50Uvavanyo ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase
6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) 48Uvavanyo
Ulawulo Olulungileyo 1 * 0.2ml ityhubhu (lyophilized)  10Uvavanyo

I-Plasmid equlethe i-Norovirus (GⅠ) amaqhekeza athile

Isisombululo sokunyibilika 1.5 ml Cryotube 500uL /
Ulawulo olubi 1.5 ml Cryotube 200uL 0.9%NaCl

Ugcino kunye noBomi beShelufu

(1)Ikhithi inokuthuthwa kubushushu begumbi.

(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.

(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.

4

Izixhobo zomculo

GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.

Umzobo wokuSebenza

a) Uguqulelo lwebhotile:

1

b) 8 inguqulelo yetyhubhu enemicu entle:

2

Ukwandiswa kwePcr

KucetyiswaUkumisela

Inyathelo Umjikelo Iqondo lobushushu (℃) Ixesha Itshaneli yeFluorescence
1 1 50 8min  
2 1 95 2 min  
3 40 95 5s  
60 10s Qokelela i-FAM fluorescence

*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.

Iziphumo zovavanyo lokutolika

Umjelo

Ukutolikwa kweziphumo

FAM Channel

Ct≤35

Norovirus (GⅠ) Positive

Undet

Norovirus (GⅠ) Negative

35

Ukuphinda kurhanelwe, phinda uvavanye*

*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.


  • Ngaphambili:
  • Okulandelayo:

  • Iimveliso ezinxulumeneyo