I-Listeria monocytogenes yePCR yokuFumana iKit

Inkcazelo emfutshane:

IListeria monocytogenes yi gram-positive microbacteria enokukhula phakathi kwe 4℃ kunye ne 45℃.Yenye yeentsholongwane eziphambili ezisongela impilo yabantu ngokutya okufakwe efrijini.


Iinkcukacha zeMveliso

Iithegi zeMveliso

Igama lemveliso

I-Listeria monocytogenes yePCR yokuFumana iKit

Ubungakanani

48tests/kit, 50tests/kit

Ukusetyenziswa okucetywayo

IListeria monocytogenes yi gram-positive microbacteria enokukhula phakathi kwe 4℃ kunye ne 45℃.Yenye yeentsholongwane eziphambili ezisongela impilo yabantu ngokutya okufakwe efrijini.Iimpawu eziphambili zosulelo yi-septicemia, i-meningitis kunye ne-mononucleosis.Le khithi ilungele ukufunyaniswa okusemgangathweni kwe-Listeria monocytogenes ekutyeni, iisampulu zamanzi, ilindle, ukuhlanza, ulwelo oluphucula ibhaktheriya kunye nezinye iisampulu kusetyenziswa umgaqo we-PCR wexesha lokwenyani le-fluorescence. , equlethe i-enzyme yokukhulisa i-DNA, i-reverse transcriptase, i-reaction buffer, i-primers ethile kunye ne-probes efunekayo ukufumanisa i-PCR ye-fluorescent.

Ugcino kunye noBomi beShelufu

(1)Ikhithi inokuthuthwa kubushushu begumbi.

(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.

(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.

4

Imixholo yeMveliso

Amacandelo Iphakheji iinkcukacha Isithako
IListeria monocytogenes PCR Mix 1 × ibhotile (Lyophilized powder)  50Uvavanyo ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase
6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) 48Uvavanyo
Ulawulo Olulungileyo 1 * 0.2ml ityhubhu (lyophilized)  10Uvavanyo

I-Plasmid equlethe i-Listeria monocytogenes amaqhekeza athile

Isisombululo sokunyibilika 1.5 ml Cryotube 500uL /
Ulawulo olubi 1.5 ml Cryotube 200uL 0.9%NaCl

Izixhobo zomculo

GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.

Umzobo wokuSebenza

a)

1

b)

2

Ukwandiswa kwePcr

KucetyiswaUkumisela

Inyathelo Umjikelo Iqondo lobushushu (℃) Ixesha Itshaneli yeFluorescence
1 1 50 8min  
2 1 95 2 min  
3 40 95 5s  
60 10s Qokelela i-FAM fluorescence

*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.

Iziphumo zovavanyo lokutolika

Umjelo

Ukutolikwa kweziphumo

FAM Channel

Ct≤35

IListeria monocytogenes Ilungile

Undet

I-Listeria monocytogenes Engalunganga

35

Ukuphinda kurhanelwe, phinda uvavanye*

*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.


  • Ngaphambili:
  • Okulandelayo:

  • Iimveliso ezinxulumeneyo