中文Ikhithi yokubona intsholongwane yorhudo lwentsholongwane yehagu ye-RT-PCR
Igama lemveliso
Intsholongwane yorhudo lwentsholongwane yehagu ye-RT-PCR (i-Lyophilized)
Ubungakanani
48tests/kit, 50tests/kit
Ukusetyenziswa okucetywayo
Le kit isebenzisa indlela yokwenyani ye-RT-PCR ye-fluorescent ukukhangela i-RNA ye-Porcine epidemic diarrhea virus (PEDV) kwizixhobo zesifo somzimba ezifana neetoni, i-lymph nodes kunye ne-spleen kunye nezixhobo zesifo solwelo ezifana nogonyo kunye negazi lehagu.Ilungele ukufunyaniswa, ukuxilongwa kunye nophando lwe-epidemiological ye-Porcine epidemic virus yorhudo.Ikhithi YINKQUBO YEPCR EZILUNGILEYO ZONKE (i-Lyophilized), equlethe i-reverse transcriptase, i-enzyme yokukhulisa i-DNA, i-reaction buffer, iiprimers ezithile kunye ne-probes ezifunekayo ukufunyanwa kwe-fluorescent RT-PCR.
Imixholo yeMveliso
| Amacandelo | Iphakheji | iinkcukacha | Isithako |
| I-PEDV PCR Mix | 1 × ibhotile (Lyophilized powder) | 50Uvavanyo | ii-dNTPs, iMgCl2, Iiprimers, Probes, Taq DNA polymerase |
| 6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) | 48Uvavanyo | ||
| Ulawulo Olulungileyo | 1 * 0.2ml ityhubhu (lyophilized) | 10Uvavanyo | IPlasmid okanye iPseudovirus equlethe amaqhekeza athile e-PEDV |
| Isisombululo sokunyibilika | 1.5 ml Cryotube | 500uL | / |
| Ulawulo olubi | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Ugcino kunye noBomi beShelufu
(1)Ikhithi inokuthuthwa kubushushu begumbi.
(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.
(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.
4
Izixhobo zomculo
GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.
Umzobo wokuSebenza
a) Uguqulelo lwebhotile:
b) 8 inguqulelo yetyhubhu enemicu entle:
Ukwandiswa kwePcr
Ummiselo oCetyisiweyo
| Inyathelo | Umjikelo | Iqondo lobushushu (℃) | Ixesha | Itshaneli yeFluorescence |
| 1 | 1 | 48 | 8min | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Qokelela i-FAM fluorescence |
*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.
Iziphumo zovavanyo lokutolika
| Umjelo | Ukutolikwa kweziphumo |
| FAM Channel | |
| Ct≤35 | I-PEDV Positive |
| Undet | I-PEDV iNegative |
| 35 | Iziphumo ezirhanelwayo, phinda uvavanye* |
*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.
