中文I-E.coli O157:Ikhithi yokubona i-PCR ye-H7
Igama lemveliso
I-E.coli O157:Ikiti yokufumanisa i-PCR ye-H7 (Lyophilized)
Ubungakanani
48tests/kit, 50tests/kit
Ukusetyenziswa okucetywayo
I-Escherichia coli O157:H7 (E.coli O157:H7) yintsholongwane yegram-negative yohlobo lwe-Enterobacteriaceae, evelisa isixa esikhulu se-Vero toxin.Kwezonyango, ngokuqhelekileyo kwenzeka ngequbuliso kunye neentlungu ezibuhlungu zesisu kunye norhudo olunamanzi, lulandelwa lurhudo olophayo kwiintsuku ezimbalwa kamva, olunokukhokelela kumkhuhlane okanye ukungabikho komkhuhlane, kunye nokufa kwiimeko ezinzima.Le khithi ifanelekile ukufunyaniswa komgangatho we-Escherichia coli O157: H7 ekutyeni, iisampulu zamanzi, ilindle, ukuhlanza, ulwelo oluphucula ibhaktheriya kunye nezinye iisampuli usebenzisa umgaqo we-PCR yexesha lokwenyani. I-Lyophilized), equlethe i-enzyme yokukhulisa i-DNA, i-reaction buffer, iiprimers ezithile kunye ne-probes ezifunekayo ukuze kubonwe i-PCR ye-fluorescent.
Imixholo yeMveliso
| Amacandelo | Iphakheji | iinkcukacha | Isithako |
| E.coli O157:H7 PCR Mix | 1 × ibhotile (Lyophilized powder) | 50Uvavanyo | ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase |
| 6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) | 48Uvavanyo | ||
| Ulawulo Olulungileyo | 1 * 0.2ml ityhubhu (lyophilized) | 10Uvavanyo | I-Plasmid equlethe i-E.coli O157: amaqhekeza athile e-H7 |
| Isisombululo sokunyibilika | 1.5 ml Cryotube | 500uL | / |
| Ulawulo olubi | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Ugcino kunye noBomi beShelufu
(1)Ikhithi inokuthuthwa kubushushu begumbi.
(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.
(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.
4
Izixhobo zomculo
GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.
Umzobo wokuSebenza
a) Uguqulelo lwebhotile:
b) 8 inguqulelo yetyhubhu enemicu entle:
Ukwandiswa kwePcr
KucetyiswaUkumisela
| Inyathelo | Umjikelo | Iqondo lobushushu (℃) | Ixesha | Itshaneli yeFluorescence |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Qokelela i-FAM fluorescence |
*Qaphela: Umqondiso wetshaneli ye-FAM fluorescence iya kuqokelelwa kuma-60℃.
Iziphumo zovavanyo lokutolika
| Umjelo | Ukutolikwa kweziphumo |
| FAM Channel | |
| Ct≤35 | E.coli O157:H7 Positive |
| Undet | E.coli O157:H7 Negative |
| 35 | Ukuphinda kurhanelwe, phinda uvavanye* |
*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.
