中文I-Listeria monocytogenes yePCR yokuFumana iKit
Igama lemveliso
I-Listeria monocytogenes yePCR yokuFumana iKit
Ubungakanani
48tests/kit, 50tests/kit
Ukusetyenziswa okucetywayo
IListeria monocytogenes yi gram-positive microbacteria enokukhula phakathi kwe 4℃ kunye ne 45℃.Yenye yeentsholongwane eziphambili ezisongela impilo yabantu ngokutya okufakwe efrijini.Iimpawu eziphambili zosulelo yi-septicemia, i-meningitis kunye ne-mononucleosis.Le khithi ilungele ukufunyaniswa okusemgangathweni kwe-Listeria monocytogenes ekutyeni, iisampulu zamanzi, ilindle, ukuhlanza, ulwelo oluphucula ibhaktheriya kunye nezinye iisampulu kusetyenziswa umgaqo we-PCR wexesha lokwenyani le-fluorescence. , equlethe i-enzyme yokukhulisa i-DNA, i-reverse transcriptase, i-reaction buffer, i-primers ethile kunye ne-probes efunekayo ukufumanisa i-PCR ye-fluorescent.
Ugcino kunye noBomi beShelufu
(1)Ikhithi inokuthuthwa kubushushu begumbi.
(2)Ubomi beshelufu ziinyanga ezili-18 ku--20℃ kunye neenyanga ezili-12 ku-2℃~30℃.
(3)Jonga ileyibhile kwikhithi ngomhla wokuveliswa kunye nomhla wokuphelelwa.
4
Imixholo yeMveliso
| Amacandelo | Iphakheji | iinkcukacha | Isithako |
| IListeria monocytogenes PCR Mix | 1 × ibhotile (Lyophilized powder) | 50Uvavanyo | ii-dNTPs, iMgCl2, Iiprimers, Probes,Reverse Transcriptase,Taq DNA polymerase |
| 6 × 0.2ml 8 ityhubhu ehlulwe kakuhle(I-Lyophilized) | 48Uvavanyo | ||
| Ulawulo Olulungileyo | 1 * 0.2ml ityhubhu (lyophilized) | 10Uvavanyo | I-Plasmid equlethe i-Listeria monocytogenes amaqhekeza athile |
| Isisombululo sokunyibilika | 1.5 ml Cryotube | 500uL | / |
| Ulawulo olubi | 1.5 ml Cryotube | 200uL | 0.9%NaCl |
Izixhobo zomculo
GENECHECKER UF-150, UF-300 real-time fluorescence PCR isixhobo.
Umzobo wokuSebenza
a)
b)
Ukwandiswa kwePcr
KucetyiswaUkumisela
| Inyathelo | Umjikelo | Iqondo lobushushu (℃) | Ixesha | Itshaneli yeFluorescence |
| 1 | 1 | 50 | 8min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Qokelela i-FAM fluorescence |
*Qaphela: Iimpawu ze-FAM fluorescence channels ziya kuqokelelwa kuma-60℃.
Iziphumo zovavanyo lokutolika
| Umjelo | Ukutolikwa kweziphumo |
| FAM Channel | |
| Ct≤35 | IListeria monocytogenes Ilungile |
| Undet | I-Listeria monocytogenes Engalunganga |
| 35 | Ukuphinda kurhanelwe, phinda uvavanye* |
*Ukuba isiphumo sovavanyo kwakhona lwetshaneli ye-FAM sinexabiso le-Ct ≤40 kwaye sibonisa igophe lemilo yokukhulisa i-“S” eqhelekileyo, isiphumo sitolikwa njenge-positive, kungenjalo sibi.
